Journal: PLoS ONE

Article Title: Induction of Autophagy by a Novel Small Molecule Improves Aβ Pathology and Ameliorates Cognitive Deficits

doi: 10.1371/journal.pone.0065367

Figure Lengend Snippet: A, SH-SY5Y/LC3-GFP and MC65 cells were treated with 20 µM GTM-1 for 12 hrs. Next, the cell lysates were analyzed using western blotting with anti-LC3 and anti-β-tubulin (as a control). B, Left: SH-SY5Y and MC65 cells were treated with 20 µM GTM-1 for 6 hrs. Next, the cell lysates were analyzed using western blotting with anti-LC3 and anti-β-tubulin (as a control). Right: quantification of the Western blot images of the ratio between LC3-II and tubulin. C, MC65 cells were treated with vehicle control (1% DMSO) or GTM-1 (20 µM) for 8 hrs. The cells were then fixed with glutaraldehyde and prepared for EM analysis. Bar, 1∶11,000. Arrows indicate double and multi-membrane autophagosomic vesicles.

Article Snippet: Rabbit polyclonal antibody anti-LC3 and mouse monoclonal antibody anti-β-tubulin were obtained from Sigma.

Techniques: Western Blot

Journal: Evidence-based complementary and alternative medicine : eCAM

Article Title: Tetrandrine-Induced Autophagy in MDA-MB-231 Triple-Negative Breast Cancer Cell through the Inhibition of PI3K/AKT/mTOR Signaling.

doi: 10.1155/2019/7517431

Figure Lengend Snippet: Figure 4: Comparison of p62/SQSTM1, Beclin1, and LC3 in MDA-MB-231 cells intervened with different concentrations of tetrandrine.

Article Snippet: Rabbit anti-LC3 Polyclonal Antibody (Cat. No: 12135-1-AP), Rabbit anti-p62/SQSTM1 Polyclonal Antibody (Cat. No: 18420-1-AP), Rabbit anti-Beclin1 Polyclonal Antibody (Cat. No: 11306-1-AP), Mouse anti-PTEN Monoclonal Antibody (Cat. No: 60300-1-Ig),Mouse anti-PI3K p85 (alpha) Monoclonal Antibody (Cat. No: 60225-1-Ig), Rabbit antiPI3K p110 (alpha) Polyclonal Antibody (Cat. No: 21890- 1-AP), Mouse anti-AKT Monoclonal Antibody (60203-2- Ig), and Mouse anti-beta Actin Monoclonal antibody (Cat. No: 66009-1-Ig) were purchased from Proteintech Group, Inc. (Chicago, USA).

Techniques: Comparison

Journal: BioMed Research International

Article Title: Neuroprotective Effect of Simvastatin via Inducing the Autophagy on Spinal Cord Injury in the Rat Model

doi: 10.1155/2015/260161

Figure Lengend Snippet: The western blot was utilized to detect the expression of mTOR, p-p70S6K, Beclin-1, and LC3-B at 3, 5, and 7 d after SCI (a). (b), (c), (d), and (e), respectively, showed the average relative gray of mTOR, p-p70S6K, Beclin-1, and LC3-B compared to the β -actin protein. Data are expressed as the mean ± SD ( n = 4/group, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001).

Article Snippet: Subsequently, the membranes were incubated at 4°C overnight with primary antibodies including anti-mTOR antibody (1 : 1000; Novus Biologicals, USA), anti-phospho-p70SK antibody (1 : 500; Abcam, Cambridge, UK), anti-LC3 antibody (1 : 1000; Novus Biologicals, USA), anti-Beclin-1 antibody (1 : 1000; Abcam), anti-brain-derived neurotrophic factor (BDNF) antibody (1 : 1000; Novus Biologicals, USA), antiglial cell line-derived neurotrophic factor (GDNF) antibody (1 : 1000; Novus Biologicals, USA), and anti- β -actin antibody (1 : 1000; Abcam, Cambridge, UK).

Techniques: Western Blot, Expressing